ABSTRACT
<p><b>OBJECTIVE</b>To research the expectorant components in volatile oil from the root and rhizome of Aster tataricus.</p><p><b>METHOD</b>GC-MS was applied to isolate and identify the compounds. In addition, TLC was used to isolate compound, and its structure was elucidated on the basis of spectral data analysis. At the same time, its expectorant effect was observed by method of the excretion quantity of phenol red in trachea of mice.</p><p><b>RESULT</b>Seven compounds were isolated and identified by GC-MS, they were (R)(-)-p-menth-1-en-4-ol (1), 2-undecanone (2), n-decanoic acid (3), (-)-spathulenol (4), hexahydrofamrnesyl acetone (5), hexadecanoic acid (6), and cis-9, cis-12-octaecadienoic acid (7). A known compound 1-acetoxy-2-ene(E)-4,6- decandiyne was isolated from the root and rhizome of A. tataricus, and it was shown to have expectorant effect.</p><p><b>CONCLUSION</b>1-Acetoxy-2-ene(E) -4,6- decandiyne, a main compound in volatile oil, had been found to have expectorant effect.</p>
Subject(s)
Animals , Male , Mice , Aster Plant , Chemistry , Drugs, Chinese Herbal , Chemistry , Pharmacology , Expectorants , Chemistry , Pharmacology , Gas Chromatography-Mass Spectrometry , Magnetic Resonance Spectroscopy , Oils, Volatile , Chemistry , Plant Roots , Chemistry , Rhizome , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC fingerprint for quantitative analysis of the active constituents of jizhi syrup.</p><p><b>METHOD</b>HPLC analysis was performed on a Zorbax Sb C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase in gradient elution was composed of (A) 1% acetic acid solution (including 0.2% triethylamine) and (B) acetonitrile, at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the wavelength was 280 nm.</p><p><b>RESULT</b>21 common peaks were tested in 10 batches of samples. Compared with the standard fingerprint, the similarity of each sample was greater than 0.99. At the same time, protocatechuic acid, protocatechu aldehyde, chlorogenic acid, caffeic acid, naringin and neohesperidin were found in all samples.</p><p><b>CONCLUSION</b>The established method can be used for the quality control of jizhi syrup.</p>
Subject(s)
Antitussive Agents , Chemistry , Benzaldehydes , Catechols , Chlorogenic Acid , Chromatography, High Pressure Liquid , Methods , Drug Combinations , Drugs, Chinese Herbal , Chemistry , Flavanones , Hydroxybenzoates , Plants, Medicinal , Chemistry , Reproducibility of ResultsABSTRACT
<p><b>OBJECTIVE</b>To establish a GC fingerprint of the volatile oil of Houttuynia cordata.</p><p><b>METHOD</b>The volatile oil was extracted from H. cordata by water stream distillation method, and analyzed by GC coupled with FID.</p><p><b>RESULT</b>12 bathes of samples collected from different regions were analyzed; the GC fingerprint of the volatile oil of H. cordata was subsequently established.</p><p><b>CONCLUSION</b>The established GC fingerprint can be used for the identification of H. cordata.</p>